1,000 trees could be cut down in Shankill to make way for the Bus Connects new number 13 route from Bray to the City Centre.
The National Transport Authority says around 330 roadside trees will have to be removed to accommodate the new bus corridor.
However residents believe the number of trees lost could be three times that figure, because it doesn't take into account trees in front gardens that may be acquired for the new bus route.
More than 2,500 residents have so far signed a petition opposing NTA's plans.
They claim that the bus route could be catastrophic for the village.