The Home from Home Creche in Greystones, County Wicklow will have to close within 21 days.
The Oblate Fathers Community Crčche in Dublin 8 will also have to close within 21 days.
As the Dublin crčche is a non-profit serving a highly disadvantaged area, steps are being taken to potentially keep it running under new management.
Tusla says it acknowledges the impact the closures will have on parents, children and staff, but added both providers were given every opportunity to make improvements.
The department says it’s been working with the Wicklow County Childcare Committee to offer support to families.
Nearly 1,000 Wicklow Children in Oversized Primary School Classes, TD Reveals
Uisce Éireann Warning As Six-Week Hosepipe Ban Takes In Stretches Along The N11 In Wicklow
Pride Of A County - Comeback Kings Wicklow Overcome 12-Point Mountain To End Wait For National Glory
No Changes To Starting Team As Wicklow Football Yearns For Historic Croker Triumph
Wicklow Braced Once More As Coillte Signal Orange Fire Warning